Identification of cis-acting repressive sequences within the negative regulatory element of human immunodeficiency virus type 1
نویسندگان
چکیده
منابع مشابه
cis-acting sequences involved in human immunodeficiency virus type 1 RNA packaging.
We have previously described a series of human immunodeficiency virus type 1-based vectors in which efficient RNA encapsidation appeared to correlate with the presence of a 1.1-kb env gene fragment encompassing the Rev-responsive element (RRE). In this report, we explore in detail the role of the RRE and flanking env sequences in vector expression and RNA encapsidation. The analysis of a new se...
متن کاملA cis-acting repressive sequence that overlaps the Rev-responsive element of human immunodeficiency virus type 1 regulates nuclear retention of env mRNAs independently of known splice signals.
The Rev protein of human immunodeficiency virus type 1 (HIV-1) binds to an RNA structure, the Rev-responsive element (RRE), to enhance expression of the viral structural genes by relieving the nuclear sequestration of incompletely spliced viral transcripts. It has been suggested that nuclear retention of these mRNAs, in mammalian cells, is due to the activity of either cis-acting repressive seq...
متن کاملIdentification of positive and negative splicing regulatory elements within the terminal tat-rev exon of human immunodeficiency virus type 1.
The requirement of human immunodeficiency virus type 1 to generate numerous proteins from a single primary transcript is met largely by the use of suboptimal splicing to generate over 30 mRNAs. To ensure that appropriate quantities of each protein are produced, there must be a signal(s) that controls the efficiency with which any particular splice site in the RNA is used. To identify this contr...
متن کاملIdentification of benzo[a]pyrene-inducible cis-acting elements within c-Ha-ras transcriptional regulatory sequences.
Previous studies in this laboratory have demonstrated that transcriptional deregulation of c-Ha-ras expression is associated with the induction and maintenance of proliferative vascular smooth muscle cell (SMC) phenotypes by benzo[a]pyrene (BaP). We examined previously undescribed cis-acting elements within the proximal 5' regulatory region of c-Ha-ras (-550 to +220) for their ability to influe...
متن کاملIntragenic cis-acting art gene-responsive sequences of the human immunodeficiency virus.
The art gene product of the human immunodeficiency virus is required for the expression of virion capsid and envelope gene proteins. The experiments presented here show that sequences located within the coding region of the envelope gene exert a negative effect on the expression of heterologous genes and that the negative effect of these sequences can be relieved by the art gene product. This r...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1990
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.64.10.5226-5229.1990